raptors

2013年2月26日 - 必应词典为您提供raptors的释义,美[ˈræptər],英[ˈræptə(r)],n. 猛禽;攫禽; 网络释义: 多伦多暴龙;猛龙;多伦多猛龙队;

2003年11月27日 - 海词词典,最权威的学习词典,为您提供raptors的在线翻译,raptors是什么意思,raptors的真人发音,权威用法和精选例句等。 raptors 英美 猛禽 Raptors的相...

目的 探讨Raptor在乳腺癌侵袭与转移过程中的作用.方法 采用Western blot检测乳腺癌细胞MCF-7和MDA-MB-231(MDA231)中Raptor蛋白的表达情况.应用小干扰RNA技术下调乳腺癌细胞MDA231中Raptor的表达量,同时将过表达质粒转入乳腺癌细胞MCF-7中,应用Western blot法检测转染后各组细胞中上皮性钙黏着蛋白(E-cadherin)和波形蛋白(Vimentin)的表达;应用Transwell体外侵袭实验检测转染后各组细胞的侵袭能力;应用细胞核质分离实验检测转染后各组细胞中Snail蛋白的转核情况.结果 MCF-7细胞中Raptor蛋白的表达量明显低于MDA231细胞中的表达量;转染后,与对照组Scr/MDA231相比,siRaptor/MDA231组中Raptor蛋白的表达量明显降低,Vimentin蛋白表达量降低,而E-cadherin 蛋白表达量升高;与对照组MCF-7/Con相比,MCF-7/Raptor组中Raptor蛋白的表达量明显升高,并伴有Vimentin蛋白表达量升高,而E-cadherin 蛋白表达量降低.siRaptor/MDA231细胞组中穿过Matrigel小室的细胞数量明显减少(P<0.05);MCF-7/Raptor细胞组中穿过Matrigel小室的细胞数量明显升高(P<0.05).细胞核质分离实验结果显示,siRaptor/MDA231组中Snail蛋白表达量较Scr/MDA231组中明显降低;MCF-7/Raptor组中Snail蛋白表达量较MCF-7/Con组中明显升高.结论 Raptor能够促进乳腺癌EMT的发生,对乳腺癌的侵袭与转移有重要作用.%Aim To study the molecular mechanism of Raptor in the migration and invasion of breast cancer cells and provide the clinical theory basis for prevention of breast cancer invasion and metastasis.Methods Western blot was used to detect the expression of Raptor protein in MCF-7 cells and MDA231 cells.The siRNA plasmids were used to transfect MDA231 cells.At the same time,the plasmid pcDNA3.1-Raptor was transfected into MCF-7 breast cancer cells.And Western blot was used to analyze the protein expression level of E-cadherin and Vimentin.Transwell was used to test the ability of invasion.Nucleus mass separation experiment was used to test the expression of Snail.Results The expression of Raptor protein in MDA231 cells was higher than in the MCF-7 cell.When the control group of Scr/MDA231 cells compared with siRaptor/MDA231,Raptor protein expression was decreased obviously after plasmid transfection interference,accompanied by reduction of Vimentin protein expression and increase of E-cadherin protein expression.Compared with MCF-7/Con,Raptor protein expression significantly increased in MCF-7/Raptor,accompanied by increase of Vimentin protein expression and reduction of E-cadherin protein expression.The number of cells through the artificial basilemma Transwell in siRaptor/MDA231 cells was significantly reduced(P<0.05),and the number of cells through the Transwell chambers artificial basilemma was significantly increased in MCF-7/Raptor(P<0.05).Nucleus mass separation experiment results showed that the expression of Snail decreased obviously in the siRaptor/MDA231 than in the Scr/MDA231,however,the expression of Snail was obviously higher in the MCF-7/Raptor.Conclusions Raptor can promote the occurrence of EMT in breast cancer,thus it promotes invasion and metastasis of breast cancer.

免疫组化检测Raptor和Rictor在慢性前列腺炎(CP)及前列腺癌(PCa)中的表达.Raptor和Rictor在CP和PCa组织中均有不同程度的表达;与CP组织比较,PCa中Raptor 和Rictor的表达均明显升高,差异有统计学意义(P<0.05).Raptor和Rictor在CP恶变为PCa过程中具有重要的作用,为PCa的预防和分子靶向治疗提供理论依据.

目的 检测Raptor、Rictor与血管生成相关因子HIF-1 α、HIF-2α以及VEGF在结直肠癌中的表达,探讨Raptor、Rictor与结直肠癌血管生成的相关性及其临床意义.方法 采用免疫组化、Western blot、RT-PCR法检测120例结直肠癌组织及60例正常结直肠黏膜组织中Raptor、Rictor及HIF-1α、HIF-2α、VEGF的表达及其差异;CD34标记微血管密度(microvascular density,MVD);分析各指标间的相关性以及与结直肠癌临床病理特征的关系.结果 Raptor、Rictor、HIF-1 α、HIF-2α和VEGF蛋白在结直肠癌中的阳性率均明显高于正常结直肠黏膜组织(P<0.05).Raptor、Rictor在中、低分化结直肠癌中的表达高于高分化结直肠癌(P<0.05),同时在淋巴结转移组的表达高于无淋巴结转移组(P <0.05);HIF-1o、HIF-2α和VEGF在淋巴结转移组的表达高于无淋巴结转移组(P<0.05);Raptor、Rictor阳性的结直肠癌组织中MVD明显高于Raptor、Rictor阴性的结直肠癌组织(P<0.05);Raptor与HIF-1α、VEGF在结直肠癌中表达呈正相关(P<0.01),Rictor与HIF-2α、VEGF表达呈正相关(P<0.01),Raptor与Rictor在结直肠癌中表达呈负相关(P<0.01).结论 mTOR核心分子Raptor、Rictor与结直肠癌的发生、发展及血管生成密切相关,两者以不同途径协同促进结直肠癌的血管生成.%Purpose To detect the expression of Raptor,Rictor,angiogenesis-related factors HIF-lα,HIF-2α and VEGF and to investigate their relationship and significance in eolorectal cancer (CRC).Methods Immunohistochemistry,Western blot and RT-PCR were employed to detect the expression of Raptor,Rictor,HIF-lα,HIF-2α and VEGF in 120 cases of CRC and 60 cases of normal colorectal mucosa.CD34 labeled microvascular density (MVD) was also observed.The correlations between Raptor,Rictor,HIF-1α,HIF-2c,VEGF expression and the patients' clinicopathological features were analyzed.Results The positive rates of Raptor,Rictor,HIF-1c,HIF-2α and VEGF in CRC were significantly higher than those in normal colorectal mucosa (P < 0.05).Raptor and Rictor expression was correlated with the degree of tumor diffcrentiation and lymph node metastasis,respectively.The expression of HIF-1α,HIF2α and VEGF was higher in patients with lymph node metastasis than those in patients without lymph node metastasis (P <0.05).The MVD was higher in patients with Raptor or Rictor positive than that in patients with Raptor or Rictor negative (P <0.05).The expression of Raptor was positively correlated with HIF-1α and VEGF (P < 0.01),the expression of Rictor was positively correlated with HIF-2o and VEGF (P < 0.01),but the expression of Raptor was negatively correlated with Rictor (P<0.01).Conclusion The expression of mTOR core molecules Raptor and Rictor is related to the initiation and development of colorectal cancer and angiogenesis,and they promote angiogenesis in colorectal cancer by different ways.

简介:此文档是关于raptors的口号的docx文档,编号为527467886,其中主题是关于经济管理、企业制度的内容展示: aptos的口号1飞跃极限,Raptos战无不胜2激情翱翔,Raptos勇往直前3翱翔蓝天,Raptos展翅高飞4雷霆出击,Raptos王者归来5挑战自我,Raptos永不言败6风驰电掣,Raptos速度制胜7飞越挑战,Raptos无畏向前8挑战巅峰,Raptos一往无前9

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